Towards optoelectronic urea biosensors
نویسندگان
چکیده
Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.
منابع مشابه
Comparison of Urea Determination in Biological Samples by Enfets Based on pH and pNH4 Detection
In this paper, urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) sensitive to ammonium ions were used. Results concerning urea determination by the biosensors in blood plasma and in dialysate show that the biosensors can be applied for urea monitoring in th...
متن کاملCarbon Nanotubes-Based Potentiometric Bio-Sensors for Determination of Urea
The possibility of using disposable plastic-carbon potentiometric sensors as enzyme biosensors was examined. Urease enzyme was immobilized on poly(vinyl chloride) based Hor NH4-selective membranes using cellulose acetate. This approach has resulted in a potentiometric response on changing the pH of the solution or NH4 ion content due to an enzymatic reaction that occurs between urease and urea....
متن کاملUrea Biosensor based on Conducting Polymer Transducers
Urea is one of the final products of protein metabolism. In clinical analysis and dairy industry urea is a very significant parameter as excess in blood serum from its permissible range causes dysfunction of the kidney. Hence, its analysis is significant and is carried out frequently in various laboratories [1, 2]. Urea is an omnipresent compound present in blood and various organic fluids. It ...
متن کامل